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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
doi: 10.3791/60571
Figure Lengend Snippet: Materials
Article Snippet: Stemdiff Neural Progenitor medium ,
Techniques: Cell Culture, Knock-Out, Membrane
Journal: Journal of Cancer
Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells
doi: 10.7150/jca.127292
Figure Lengend Snippet: Human Neural Progenitor Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.
Article Snippet: Neural Progenitor Cells (ACS-5004),
Techniques: Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software, Positive Control
Journal: Journal of Cancer
Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells
doi: 10.7150/jca.127292
Figure Lengend Snippet: Human neural progenitor cells (NPCs) display reduced neurite outgrowth with exosomes and CM from C4-2 Snail knockdown (C4-2 E8) cells compared to C4-2 Non-silencing (NS) cells. C4-2 NS or C4-2 E8 was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes, or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (B) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (C) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.
Article Snippet: Neural Progenitor Cells (ACS-5004),
Techniques: Knockdown, Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software